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KMID : 0363020050350020345
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Journal of Korean Academy of Periodontology
2005 Volume.35 No. 2 p.345 ~ p.357
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The effect of rhBMP-2 on △(12)-PGJ2 induced osteoblastic differentiation and mineralization
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김원경
김경화/김종진/이영규/구영
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Abstract
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Prostaglandin plays a significant role in the local control of bone metabolism associated
with periodontal disease. Δ
12-PGJ2 is a natural PGD2 metabolite that is formed in vivo in the
presence of plasma. It is known for Δ
12-PGJ2 to stimulate calcification in osteoblastic cells.
Bone morphogenetic protein(BMP) stimulated osteoblastic differentiation in various types of
cells and greatly enhanced healing of bony defects.
The purpose of this study was to evaluate the effect of rhBMP-2 on Δ
12-PGJ2 induced
osteoblastic differentiation and mineralization in vitro. A human osteosarcoma cells line Saos-2
were cultured. In the test groups, 10-7M of Δ
12-PGJ2 or mixture of 10-8M of Δ
12-PGJ2 and
100ng/ml of rhBMP-2 or 100ng/ml of rhBMP-2 were added to culture media. After 1 day, 2
days and 4 days of culture period, the cell number was measured. Alkaline phosphatase
activity was measure at 3 days. Reverse transcription polymerase chain reaction(RT-PCR)
was performed to determine the expression of mRNA of bone matrix protein at 8 hours, 1
day and 7 days. The ability to produce mineralized nodules in rat osteoblasts(MC3T3-E1) was
evaluated at 21 days.
The results were as follows :
1. rhBMP-2 or mixture of rhBMP-2 and Δ
12-PGJ2 inhibited cell proliferation of human
osteosarcoma cells.
2. rhBMP-2 or mixture of rhBMP-2 and Δ
12-PGJ2 stimulated alkaline phosphatase activity
significantly higher than Δ
12-PGJ2 alone.
3. rhBMP-2 or mixture of rhBMP-2 and Δ
12-PGJ2 stimulated mineralization compared to Δ
12-PGJ2 alone.
4. mRNA of alkaline phosphatase, BMP-2, cbfa 1, Type I collagen were detected in the
group treated with Δ
12-PGJ2/rhBMP-2, rhBMP-2 alone, Δ
12-PGJ2 alone.
These results show that mixture of Δ
12-PGJ2 and rhBMP-2 causes more bone formation than
Δ
12-PGJ2 alone while the bone formation effects of mixture of Δ
12-PGJ2 and rhBMP-2 are less
than those of rhBMP-2 alone.
Further researches would be necessary to clarify the interactions of these agents.18)
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KEYWORD
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BMP-2, △(12)-PGJ2, Saos-2 cell, MC3T3-E1 cell, Alkaline phosphatase activity, RT-PCR
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